Mmanụ artemisia capillaris dị ọcha maka kandụl na ncha na-eme ka diffuser n'ogbe dị mkpa mmanụ dị ọhụrụ maka ndị na-ere ọkụ ahịhịa amị.

Mmanụ artemisia capillaris dị ọcha maka kandụl na ncha na-eme nnukwu mgbasa ozi dị mkpa mmanụ ọhụrụ maka ndị na-ere ọkụ ahịhịa egosipụtara

nkowa nkenke:

Nlere anya nke rodent

E kewara anụmanụ ndị ahụ na-enweghị usoro ụzọ ise nke oke iri na ise. Ejikọtara ndị njikwa na ụdị oke okemmanụ sesamemaka ụbọchị 6. E jiri mbadamba bifendate (BT, 10 mg/kg) were kpuchie ụmụ oke na-ahụ maka njikwa dị mma maka ụbọchị 6. A na-emeso ndị otu nnwale ahụ na 100 mg / kg na 50 mg / kg AEO gbazere na mmanụ sesame maka ụbọchị 6. N'ụbọchị 6, a na-eji mmanụ sesame na-ahụ maka njikwa ahụ, na ndị ọzọ niile na-eji otu dose nke 0.2% CCl4 na mmanụ sesame (10 ml / kg) site na ya.injection intraperitoneal. A na-ebukwa ọnụ oke ndị ahụ n'enweghị mmiri, a na-anakọtakwa ọbara n'ime arịa retrobulbar; A na-etinye ọbara achịkọtara na 3000 ×gmaka 10 min iji kewaa serum.Mgbapụ nke cervicale mere ozugbo e wepụrụ ọbara, e wepụrụ ihe nlele imeju ngwa ngwa. A na-echekwa otu akụkụ nke imeju ozugbo na -20 Celsius C ruo mgbe nyochachara, ma wepụ akụkụ ọzọ ma dozie ya na 10%formalinngwọta; A na-echekwa anụ ahụ ndị fọdụrụ na -80 Celsius maka nyocha histopathological.Wang et al., 2008,Hsu et al., 2009,Nie et al., 2015).

Ntụle paramita biochemical na serum

A na-enyocha mmerụ ahụ imeju site n'ịtụle ihe ahụọrụ enzymaticnke serum ALT na AST na-eji ngwa azụmahịa dabara adaba dịka ntuziaka maka ngwa (Nanjing, Jiangsu Province, China). E gosipụtara ọrụ enzymatic dị ka nkeji kwa liter (U / l).

Ntụle nke MDA, SOD, GSH na GSH-Pxna imeju homogenate

A na-ejikọta anụ ahụ imeju na saline physiological oyi na nha 1: 9 (w / v, imeju: saline). Ndị homogenates bụ centrifuged (2500 ×gmaka 10 min) iji nakọta ndị isi maka mkpebi ndị na-esote. A na-enyocha mmebi imeju dabere na nha imeju nke ọkwa MDA na GSH yana SOD na GSH-P.xihe omume. Ekpebiri ihe ndị a niile na-eso ntuziaka dị na ngwa ahụ (Nanjing, Jiangsu Province, China). E gosipụtara nsonaazụ maka MDA na GSH dị ka protein nmol kwa mg (nmol / mg prot), yana ọrụ SOD na GSH-P.xE gosipụtara ya dị ka protein U kwa mg (U/mg prot).

Histopathological analysis

A na-edozi akụkụ nke imeju enwetara ọhụrụ na 10% echekwabaraparaformaldehydengwọta phosphate. A na-etinye ihe nlele ahụ na paraffin, bee n'ime akụkụ 3-5 μm, nke nwere ntụpọ.hematoxylinnaeosin(H&E) dị ka usoro ọkọlọtọ si dị, n'ikpeazụ wee nyochaa yaobere microscope(Tian et al., 2012).

Nyocha ndekọ ọnụ ọgụgụ

Ekwuputara nsonaazụ ya dị ka ihe pụtara ± ọkọlọtọ (SD). A tụlere nsonaazụ ya site na iji mmemme mgbakọ na mwepụ SPSS Statistics, ụdị 19.0. Edebere data ahụ na nyocha nke ọdịiche (ANOVA,p<0.05) sochiri ule Dunnett na ule Dunnett T3 iji chọpụta ọdịiche dị ịrịba ama dị n'etiti ụkpụrụ nke otu nnwale dị iche iche. A na-atụle ọdịiche dị ịrịba ama na ọkwa nkep<0.05.

Nsonazụ na mkparịta ụka

Ihe mejupụtara nke AEO

Na nyocha GC/MS, achọpụtara na AEO nwere ihe mejupụtara 25 gbapụrụ site na 10 ruo 35 min, na ihe mejupụtara 21 na-aza 84% nke mmanụ dị mkpa ka amapụtara.Tebụl 1). Mmanụ na-agbanwe agbanwe dịmonoterpenoids(80.9%), sesquiterpenoids (9.5%), hydrocarbons enweghị alaka (4.86%) na acetylene dị iche iche (4.86%). Tụnyere ọmụmụ ihe ndị ọzọ (Guo et al., 2004), anyị hụrụ ọtụtụ monoterpenoids (80.90%) na AEO. Nsonaazụ gosiri na ihe kachasị nke AEO bụ β-citronellol (16.23%). Akụkụ ndị ọzọ bụ isi nke AEO gụnyere 1,8-cineole (13.9%),camphor(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3.99%),thymol(3.22%) namyrcene(2.02%). Ọdịiche dị na kemịkalụ ahụ nwere ike jikọta ya na ọnọdụ gburugburu ebe obibi nke osisi ahụ gosipụtara, dị ka mmiri ịnweta mmiri, ìhè anyanwụ, ogbo nke mmepe naoriri na-edozi ahụ.

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Ọrịa imeju, ọrịa nkịtị kpataranje ịba ọcha n'anya, mmanya na-egbu egbu, kemịkalụ imeju na-egbu egbu, àgwà nri na-adịghị mma na mmetọ gburugburu ebe obibi, bụ nchegbu zuru ụwa ọnụ (Papay et al., 2009). Otú ọ dị, ọgwụgwọ maka ọrịa a na-esikarị ike inye ya ma nwee mmetụta a kpachiri anya. Chinese omenalaọgwụ herbal, nke dabara n'ọtụtụ ndenye ọgwụ eji agwọ ọrịa imeju, ndị China ka na-eji ya eme ihe (Zhao et al., 2014).Artemisia capillarisTnb.,Asteraceae, dị ka Bencao Gangmu si kwuo, a na-eji ihe ndekọ kachasị ama nke nkà mmụta ọgwụ ọdịnala ndị China, mee ihe n'ọtụtụ ebe dị ka ọgwụ iji kpochapụ okpomọkụ, kwalite.diuresisma wepụ jaundice ma jiri ya mee ihe dị ka ekpomeekpo na mmanya, akwụkwọ nri, na pastry n'ihi na ọ na-esi ísì ụtọ.A. capillarisọnụ ọgụgụ na-arị elu nke ndị mmadụ ewerewo dị ka ụdị ọgwụ na nri ndị China. Ya mere, enwere nnukwu mbọ iji mepụta ọgwụ ahịhịa bara uru, dịkaA. capillaris, maka ọgwụgwọ ọrịa imeju.

N'ime afọ ndị na-adịbeghị anya, ọgwụ herbal enwetawo nlebara anya na ewu ewu maka ọgwụgwọ ọrịa imeju n'ihi nchekwa na ịdị irè ha (Ding et al., 2012).A. capillarisegosila na ọ nwere ezigbo ọrụ nchekwa hepatoprotective dabere na usoro ọgwụ ọgbara ọhụrụHan et al., 2006). Ọ bụkwa ihe dị mkpa ọgwụ na China ma bụrụ ihe na-ewu ewu na-egbochi mkpali (Cha et al., 2009a),choleretic(Yoon na Kim, 2011), na mgbochi tumor (Feng et al., 2013)herbal usọbọ.

Phytochemicalọmụmụ ekpughere ọtụtụ mmanụ dị mkpa na-agbanwe agbanwe,coumarins, naflavonol glycosidesyana otu ndị na-amaghị amaaglyconessiA. capillaris(Komiya et al., 1976,Yamahara et al., 1989). Mmanụ dị mkpa nkeA. capillaris(AEO) bụ otu n'ime ndị isi ọgwụ na-arụ ọrụ ogige ma na-enye mgbochi mkpali.Cha et al., 2009ana mgbochi apoptotic Njirimara (Cha et al., 2009b). Otú ọ dị, dị ka AEO bụ otu n'ime ndị isi ogige nkeA. capillaris, ike hepatoprotective ọrụ nke isi mejupụtara siA. capillariskwesịrị inyocha.

N'ime ọmụmụ ihe a, mmetụta nchebe nke AEO nacarbon tetrachloride(CCl4) - kpaliterehepatotoxicityA tụlere ya site na usoro biochemical, dị ka ịba ọcha n'anyaglutathione belatara(GSH),malondialdehydeỌkwa (MDA),superoxide dismutase(SOD), naglutathione peroxidase(GSH-Px) ọrụ, yana ọrụ nkeaspartate aminotransferase(AST) naalanine aminotransferase(ALT) n'ime ọbara. A na-enyochakwa oke mmerụ imeju nke CCl4 kpatara site na nyocha akụkọ ihe mere eme, tinyere nyocha phytochemical site na GC-MS iji chọpụta ihe ndị mejupụtara AEO.