Osteoarthritis (OA) bụ otu n'ime ọrịa nkwonkwo ọkpụkpụ na-adịghị ala ala nke na-emetụta ndị agadi karịa 65.
1]. N'ozuzu, a na-achọpụta ndị ọrịa OA na cartilage mebiri emebi, synovium na-ere ọkụ, na chondrocytes na-emebi emebi, nke na-ebute mgbu na nhụjuanya anụ ahụ.
2]. Ihe mgbu nke arthritic na-ebutekarị site na mbelata nke cartilage na nkwonkwo site na mbufụt, na mgbe cartilage mebiri emebi nke ọma ọkpụkpụ nwere ike ịgbakọta ibe ya na-akpata mgbu na ihe isi ike anụ ahụ.
3]. Ntinye aka nke ndị ogbugbo mkpali nwere mgbaàmà dịka mgbu, ọzịza, na isi ike nke nkwonkwo na-edepụta nke ọma. N'ime ndị ọrịa OA, a na-ahụ cytokines na-egbuke egbuke, nke na-ebute mmebi nke cartilage na ọkpụkpụ subchondral n'ime mmiri synovial.
4]. Mkpesa abụọ bụ isi nke ndị ọrịa OA na-enwekarị bụ mgbu na mbufụt synovial. Ya mere, ebumnuche bụ isi nke usoro ọgwụgwọ OA ugbu a bụ iji belata mgbu na mbufụt. [
5]. Ọ bụ ezie na ọgwụgwọ OA dịnụ, gụnyere ọgwụ ndị na-abụghị steroidal na steroidal, egosila na ọ dị irè n'ibelata mgbu na mbufụt, iji ọgwụ ndị a na-adịte aka na-enwe mmetụta ahụike siri ike dị ka ọrịa obi, gastro-intestinal, na renal dysfunctions.
6]. Ya mere, a ghaghị ịmepụta ọgwụ dị irè karị nke nwere mmetụta dị ole na ole maka ọgwụgwọ ọrịa ogbu na nkwonkwo.
Ngwaahịa ahụike sitere n'okike na-ewuwanye ewu maka ịdị mma yana ịdị mfe dị [
7]. Ọgwụ ndị Korea ọdịnala egosiwo na ọ dị irè megide ọtụtụ ọrịa mkpali, gụnyere ogbu na nkwonkwo [
8]. Aucklandia lappa DC. mara maka ọgwụ ya, dị ka ịkwalite mgbasa nke qi maka ibelata mgbu na ime ka afọ dị jụụ, ma ejiri ya mee ihe n'ọdịnala dị ka analgesic eke.
9]. Akụkọ gara aga na-egosi na A. lappa nwere mgbochi mkpali [
10,
11, analgesic [
12], anticancer [
13na gastroprotective.
14] mmetụta. A na-eme ihe omume dị iche iche nke A. lappa site na ogige ya bụ isi na-arụ ọrụ: costunolide, dehydrocostus lactone, dihydrocostunolide, costuslactone, α-costol, saussurea lactone na costuslactone.
15]. Nnyocha ndị gara aga na-ekwu na costunolide gosipụtara ihe mgbochi mkpali na lipopolysaccharide (LPS), nke kpalitere macrophages site na nhazi nke NF-kB na ụzọ protein ujo ọkụ.
16,
17]. Otú ọ dị, ọ dịghị nnyocha ọ bụla enyochala ihe omume A. lappa nwere maka ọgwụgwọ OA. Nnyocha a dị ugbu a enyochala mmetụta ọgwụgwọ nke A. lappa megide OA site na iji (monosodium-iodoacetate) MIA na acetic acid-induced rodent model.
Monosodium-iodoacetate (MIA) bụ nke a ma ama iji mepụta ọtụtụ n'ime omume mgbu na njirimara pathophysiological nke OA na anụmanụ.
18,
19,
20]. Mgbe etinyere ya n'ime nkwonkwo ikpere, MIA na-akụghasị metabolism chondrocyte ma na-ebute mbufụt na mgbaàmà mkpali, dị ka cartilage na ọkpụkpụ subchondral ọkpụkpụ, ihe mgbaàmà nke OA.
18]. A na-ewere nzaghachi ederede nke sitere na acetic acid dị ka ịme anwansị nke mgbu n'akụkụ anụ ụlọ ebe enwere ike tụọ ihe mgbu na-egbu mgbu.
19]. A na-eji ahịrị cell macrophage mouse, RAW264.7, nke a na-ejikarị amụ nzaghachi cellular na mbufụt. Mgbe ejiri LPS rụọ ọrụ, RAW264 macrophages na-arụ ọrụ ụzọ mkpali ma na-ezobe ọtụtụ ndị na-eme mkpesa, dị ka TNF-α, COX-2, IL-1β, iNOS, na IL-6.
20]. Ọmụmụ ihe a enyochala mmetụta mgbochi-nociceptive na mgbochi mkpali nke A. lappa megide OA na ụdị anụmanụ MIA, ụdị anụmanụ nke acetic acid kpatara, na sel RAW264.7 na-arụ ọrụ LPS.
2. Akụrụngwa na Ụzọ
2.1. Ihe ọkụkụ
Mgbọrọgwụ akpọnwụ nke A. lappa DC. Ejiri ya mee nnwale ahụ sitere na Epulip Pharmaceutical Co., Ltd., (Seoul, Korea). Ọ bụ Prof. Donghun Lee, Dept. nke Herbal pharmacology, Col. nke Korean Medicine, Gachon University, mara ya, na ego speciment nọmba e debere dị ka 18060301.
2.2. Nyocha HPLC nke A. lappa wepụ
A na-ewepụta A. lappa site na iji ngwa reflux (mmiri distilled, 3 h na 100 Celsius). A na-enyocha ihe ngwọta nke amịpụtara ma kpochie ya site na iji evaporator dị ala. Mwepụta A. lappa nwere mkpụrụ nke 44.69% ka akpọnwụchara n'okpuru -80 Celsius. Emere nyocha Chromatographic nke A. lappa site na ijikọ HPLC site na iji 1260 InfinityⅡ HPLC-system (Agilent, Pal Alto, CA, USA). Maka nkewa chromatic, a na-eji kọlụm EclipseXDB C18 (4.6 × 250 mm, 5 µm, Agilent) na 35 Celsius. Ngụkọta 100 mg nke ihe nleba anya ka agbazere na 10 ml nke methanol 50% wee tinye ya maka nkeji iri. Ejiri nzacha sirinji (Waters Corp., Milford, MA, USA) nke 0.45 μm wee jiri nzacha sirinji kpụchaa nlele. Ngwakọta usoro mkpanaaka bụ 0.1% phosphoric acid (A) na acetonitrile (B) na kọlụm dị ka ndị a: 0-60 min, 0%; 60–65 nkeji, 100%; 65–67 nkeji, 100%; 67-72 min, 0% ihe mgbaze B nwere oke ọsọ nke 1.0 ml / min. A na-ahụ nsị na 210 nm na-eji olu injection nke 10 μL. Emere nyocha ahụ ugboro atọ.
2.3. Ụlọ na njikwa anụmanụ
Azụtara ụmụ oke Sprague – Dawley (SD) gbara izu ise na ụmụ oke ICR gbara izu isii n'aka Samtako Bio Korea (Gyeonggi-do, Korea). A na-edobe anụmanụ n'ime ụlọ na-eji okpomọkụ mgbe niile (22 ± 2 ° C) na iru mmiri (55 ± 10%) na ìhè / ọchịchịrị okirikiri nke 12/12 h. Ụmụ anụmanụ maara ọnọdụ ahụ ihe karịrị otu izu tupu nnwale ahụ amalite. Ụmụ anụmanụ nwere ihe oriri na mmiri ad libitum. A na-agbaso iwu ụkpụrụ omume ugbu a maka nlekọta na njikwa anụmanụ na Mahadum Gachon (GIACUC-R2019003) na usoro nnwale anụmanụ niile. Emebere ọmụmụ ihe ahụ-nyocha-nyocha na nnwale yiri ya. Anyị gbasoro usoro euthanasia dịka ntuziaka nke kọmitii ụkpụrụ omume nnwale anụmanụ siri dị.
2.4. MIA injection na ọgwụgwọ
E kewara oke oke na otu anọ, ya bụ sham, control, indomethacin, na A. lappa. N'ịbụ ndị ejiri 2% isofluorane O2 ngwakọta, a na-agbanye oke ndị ahụ site na iji 50 μL nke MIA (40 mg / m; Sigma-Aldrich, St. Louis, MO, USA) n'ime nkwonkwo ikpere na-eduga na OA nnwale. Emere ọgwụgwọ ndị a dị ka n'okpuru: a na-echekwa njikwa na ndị otu sham naanị site na nri nri AIN-93G. Naanị, a na-enye otu indomethacin na indomethacin (3 mg / kg) etinyere n'ime nri AIN-93G yana otu A. lappa 300 mg/kg kenyere nri AIN-93G agbakwunyere na A. lappa (300 mg / kg). A gara n'ihu na ọgwụgwọ ahụ maka ụbọchị 24 kemgbe ụbọchị ntinye OA na ọnụego 15-17 g kwa 190-210 g ịdị arọ ahụ kwa ụbọchị.
2.5. Ntụle ịdị arọ
Mgbe ntinye OA gasịrị, a na-eji ike-MeterTester600 (IITC Life Science, Woodland Hills, CA, USA) mee nha ike ibu nke akụkụ azụ nke oke ndị ahụ dịka ahaziri. A gbakọrọ nkesa ịdị arọ na ụkwụ ụkwụ azụ: ikike ibu ibu (%)
